Protective Effects Of Tecomella Undulata Stem Bark Extract On Isoniazid Induced Hepatotoxicity: Based On Liver Enzymes And Histopathology In Rat Model

Abstract

Objective: To evaluate the hepatoprotective effects of Tecomellaundulata stem bark extract on isoniazid induced hepatic damage based on liver enzymes and Liver function test in rat models. Study design and Setting: An experimental study conducted at Department of Pharmacology at Al-Tibri Medical College and Hospital, Isra University Karachi Campus and Dow University of Health Sciences, Karachi. Methodology: Total twenty-four rats were studied. The albino rats that were male, healthy, and weighing 200-250grams were included in this study. Rats were divided into four groups, each group having six rats and treated once daily orally for 30 days. Group A was control group and treated with normal animal diet and water; Group B was Isoniazid treated group and induced by oral administration of Isoniazid (INH) 50mg/kg. Group C was treated with Isoniazid 50mg/kg and Tecomellaundulata bark extract with low dose of 200mg/kg . Group D was treated with Isoniazid 50mg/kg and Tecomellaundulata bark extract with high dose of 400mg/kg . All the animals were weighed before commencement of the study. Liver enzymes were noted after the end of experiment. P value of <0.05 was taken as significant. Results: While comparing the mean values of AST,ALT, ALP and GGT in all four groups group; the statistical significant difference (p<0.001) was found. The mean levels in of total Bilirubin in group A was 0.69 ±0.01, group B 1.04 ±0.04, in group C was 1.15 ±0.39, and in group D was 1.04 ±0.44 with the significant difference (p=0.004). Conclusion: Tecomellaundulata has a protective effect on isoniazid induced toxicity on liver as evidenced by liver function test on rat models.