Abstract:
Objective: To compare the effects of drugs (Metformin and combination) on weight, size and volume of liver in diabetic
rats.
Study Design and Setting: This was an experimental comparative study conducted at Institute of Bio Medical Sciences
(IBMS), Dow University of Health Sciences (DUHS), Ojha Campus, Karachi.
Methodology: The study was conducted on 40 albino wistar rats. Diabetic rats (n=20) were grouped into 2. One group
was treated with metformin (n=10)while the other group with insulin-metformin combination (n=10) for 4 weeks. All the
treated groups were compared with untreated diabetic killed (n=10). After a short period of 4 weeks, all the rats were
sacrificed. Livers were isolated, weighed and grossly observed for significant changes. Absolute and mean percent liver
weights as well as liver volumeof all rats were calculated.
Results: Data was analyzed by using GraphPad Prism version 5.0.Data for absolute/percent liver weight and liver volume
was expressed as Mean ± SEM. The value obtained was analyzed by Two-way ANOVA followed by Bonferroni test
wherever applicable.P-value of = 0.05 was considered as statistically significant.
Conclusion: The short-term metformin and insulin-metformin treatment doesn’t effect liver weight and volume significantly
and hence, their short-term use is beneficial in type 2 diabetes
