Bioaccumulation and oxidative stress in Fish on chlorpyrifos exposure (T-1334) (MFN 8135)

Abstract

Unsustainable agricultural practices and excessive usage of pesticides have provoked the pollution problems. only 0.1% reaches to target in agroecosystem, remaining enters into different ecosystems. Extensive usage of pesticides ultimately finds their way into aquatic environment. Pesticide contains micro organic pollutants that have ecological impacts. Phosphorylation, major insecticides that are usually applied in agriculture. Chloroforms, an organophosphate pesticide is common in use to control pests. CPF, a second largest selling pesticides, enters in water body and interfere with aquatic organisms. In present study an attempt was performed to analyze toxicity of CPF with reference to fish. The objective of this study was to observe oxidative stress in silver carp. This study was conducted by exposing fish to 0.02 ����l, ��������������������l��������������������l, 20 ����l����������������������������l for 24, 48, 72 and 96 hours and along with control group. Toxicity potentials CPF was determined by analyzing CAT, GST, GSH and SOD activities in liver, gills, kidney, heart, brain and muscle. Spectrophotometric analysis revealed significant alterations in ROS production in liver, gills, brain, kidney, and heart and muscle tissue of exposed fish with elevations in concentrations and exposure duration of CPF. CAT, GST activities decreases in heart, muscle, brain, kidney, gills and liver after 24 hour exposure time period. While SOD increases till 38% (200 ����l in heart) 56% (in muscle) 31 %, 58% 48% and 59% respectively, as compared to control group. After 48 hours exposure period CAT, GSH, GST decreases in organ. SOD activities shows little elevations. After 72 hour GSH increases ( brain, liver & kidney), GST and CAT decreases with elevations in SOD activities has been found. Alterations in GST and CAT remained GSH shows fluctuations at the end of exposure. CPF induces oxidative stress and have toxic effects in fish. GC analysis of various organs shown order of accumulation of chlorpyrifos as >liver>gills>kidney>muscle. Significant accumulation of CPF has been found in muscle.