Abstract:
Objective: To evaluate the antibiotic resistance pattern of Pseudomonas aeruginosa and its prevalence from samples
received from Intensive Care Units (adult and neonatal) at PNS Shifa Hospital Karachi.
Study Design and Setting: This cross sectional study was carried out at PNS SHIFA hospital.
Methodology: Samples including blood, pus, wound swab, sputum and endobronchial washing were received for culture
and sensitivity. Isolates were cultured on blood and MacConkey agar. Antibiotic susceptibility testing was performed by
Kirby-Bauer and broth microdilution and then analyzed on SPSS version 23.Results were confirmed by VITEK 2.
Results: Out of 674 positive clinical specimens 97(14.39%) were positive for Pseudomonas aeruginosa growth. The most
susceptible antibiotic against Pseudomonas aeruginosa was Polymixin b (94.854%).The least effective antimicrobial was
aztreonam (40.21%sensitive).
Conclusion: The prevalence of Pseudomonas aeruginosa from the samples of Intensive Care Units was found to be 14.39%.
The most susceptible antibiotic against pseudomonas was Polymixin B. The least effective antimicrobial was aztreonam
