Abstract:
The search for minimally invasive treatment of osteoarthritis has led to the development of biological
options such as platelet-rich plasma (PRP), mesenchymal stem cells (MSCs), and bone marrow aspirate
concentrates. This research was conducted to study the outcomes of PRP administration in the chemicalinduced
model of osteoarthritis in rat knee.
Methods and results
Two milligrams of monoiodoacetate (MIA) was used for the induction of arthritis in the right knee of 16 rats.
Autologous PRP was prepared by double centrifugation, which was then administered in the arthritic knee of
eight rats. This group was labeled as the treated group (A) while the rest were counted as the non-treated
group (B). Chondrocyte count and uncalcified cartilage thickness were morphometrically assessed on
hematoxylin and eosin (H&E) stained slides, and it was noted that treated group A had a higher chondrocyte
count and more cartilage height as compared to non-treated group B. Intergroup comparison was done
between the treated group (A) and non-treated group (B) using the independent t-test. P-values were found
to be statistically significant for these parameters.
Conclusion
This study thus concluded that PRP had induced an inhibitory effect on the apoptosis of chondrocytes,
which, in turn, prevented the loss of cartilage height by inhibiting matrix loss.
