Ameliorative Effects of Two Forms of Pomegranate on Glomerular Transvertical Diameter in Steroid- Induced Kidney Damage in Mice

Abstract

Background: To observe the effects of Pomegranate juice (PJ) and Pomegranate peel extract (PPE) on glomerular transvertical diameter in steroid induced mice kidney damage. Methods: In this experimental study forty healthy adult mice (BALB/c strain), average weighing 25-30 gms were divided into four groups, having ten mice each. Control group A received only standard laboratory diet without alteration.Experimental groups B, C and D were injected ND (Nandrolone decanoate) (1 mg/100 gm body weight), intramuscularly (I/M), in the hind limb once a week for 8 weeks. Experimental group C was also given PJ (3ml/kg body weight) by oral gavage tube daily for 8 weeks and experimental group D was given PPE (200mg/kg body weight) through oral gavage tube,daily for 8 weeks.After the experimental period, the animals were sacrificed and both kidneys of all mice were obtained. Kidneys were processed, embedded and stained for histological study by using Hematoxylin and Eosin (H&E) and Periodic Acid Schiff (PAS) stains. The results were compiled and compared. Results: After ND administration, glomerular diameter was significantly reduced in experimental group B when compared to control group A.Protective effects were seen when comparison of experimental group B was done with PJ and PPE administered experimental groups C and D, respectively. When results of experimental groups C and D were compared with each other no statistical significance was present. Conclusion: Both forms of Pomegranate has ameliorative effects on glomerular transvertical diameter in steroid induced mice kidney disease.