Abstract:
Background: To observe the effects of
Pomegranate juice (PJ) and Pomegranate peel extract
(PPE) on glomerular transvertical diameter in steroid
induced mice kidney damage.
Methods: In this experimental study forty healthy
adult mice (BALB/c strain), average weighing 25-30
gms were divided into four groups, having ten mice
each. Control group A received only standard
laboratory diet without alteration.Experimental
groups B, C and D were injected ND (Nandrolone
decanoate) (1 mg/100 gm body weight),
intramuscularly (I/M), in the hind limb once a week
for 8 weeks. Experimental group C was also given PJ
(3ml/kg body weight) by oral gavage tube daily for 8
weeks and experimental group D was given PPE
(200mg/kg body weight) through oral gavage
tube,daily for 8 weeks.After the experimental period,
the animals were sacrificed and both kidneys of all
mice were obtained. Kidneys were processed,
embedded and stained for histological study by
using Hematoxylin and Eosin (H&E) and Periodic
Acid Schiff (PAS) stains. The results were compiled
and compared.
Results: After ND administration, glomerular
diameter was significantly reduced in experimental
group B when compared to control group
A.Protective effects were seen when comparison of
experimental group B was done with PJ and PPE
administered experimental groups C and D,
respectively. When results of experimental groups C
and D were compared with each other no statistical
significance was present.
Conclusion: Both forms of Pomegranate has
ameliorative effects on glomerular transvertical
diameter in steroid induced mice kidney disease.
