Abstract:
Objective: To determine the comparative and combined synergetic effect of Black Coffee and Metforminin treatment of type 2 diabetes mellitus in mice model.
Study Design: Experimental, Randomized control study.
Place and Duration of Study: This study was conducted over a period of one year from May 2017 to April 2018 at Pharmacology laboratory and Multidisciplinary laboratory of Islamic International Medical College in collaboration with National Institute of Health, Islamabad.
Materials and Methods: A total of 50 male Balb/c albino mice were taken, group 1 was non-diabetic normal control (n=10) and diabetes was induced in experimental group (n=40) by using low dose streptozotocin (40mg/kg). After confirmation, diabetic mice were further divided into four groups (10 mice/group). Group 2 was diabetic control and remaining 3 groups were treated with black coffee, metformin and combination of both, respectively for 45 days. Blood samples were taken by intracardiac puncture for HbA , which shows the 1C long-term control. Statistical analysis was done applying SPSS 21. Comparisons of means of HbA between the 1C groups were analyzed using one way ANOVA (post hoc tuckey test). P value of <0.05 was considered significant.
Results: Black coffee treated (Group 3), metformin treated (Group 4) and combination of black coffee and
metformin treated (Group 5) had significantly decreased serum HbA levels in comparison with those found in 1C diabetic control (Group 2) (P<0.05).
Conclusion: Combination of black coffee and metformin significantly decreases serum HbA levels in diabetic 1C mice as compared to metformin or black coffee treated diabetic mice separately.
