Abstract:
hepatotoxicity in rats.
Study Design: Experimental-randomized control study.
Place and Duration of Study: The research was conducted from October 2018 to October 2019 in
Pharmacology Department at IIMCT in mutual collaboration with National Institute of Health (NIH) Islamabad.
Material and Methods: At day 0 after initial blood sampling, Acetaminophen (300 mg/kg) by intraperitoneal
route was given to 30 rats to induce hepatotoxicity. These rats were further divided into three experimental
groups on day 8. Group 2 was a disease control group, Silymarin (100mg/kg) was given to group 3 rats and group
4 rats were treated with Coffee (200 mg/kg) through intragastric gavage for fourteen days. Terminal blood
sampling was done at day 21 through cardiac puncture for biochemical estimation on same day. Mean± SEM
was calculated and analyzed through SPSS 20. P value less than 0.05 was considered statistically significant.
Results: Our results showed major elevation (p<0.05) in alanine aminotransferase and aspartate
aminotransferase levels in group 2 when compared to normal control -group. The rats treated with silymarin &
coffee considerably (p <0.05) lowered biomarker enzymes in comparison to disease control group 2
respectively.
Conclusion: Coffee lowers ALT and AST levels as compared to Silymarin in Acetaminophen induced
hepatotoxicity in rats.
