Abstract:
Objective: To determine the sensitivity of Carbapenem-resistant Pseudomonas aeruginosa (CARP) with the panel of antibiotics
according to Clinical and Laboratory Standards Institute (CLSI) 2020 guidelines.
Study Design: Cross-sectional study.
Duration and Place of Study: Armed Forces Institute of Pathology, Rawalpindi from Pakistan, Apr to Sep 2020.
Methodology: All samples received at the Microbiology Department during the study period were included. Various samples
such as blood, pus, sputum, Endobronchial washing (EBW), non-directed bronchial lavage (NBL), tissue and urine were
processed in the laboratory. Samples were inoculated on appropriate culture media, and bacteria were identified according to
their colony morphology, Gram staining characteristics and biochemical tests. Antibiotic susceptibility testing was performed
using the Kirby Bauer Disk Diffusion technique according to Clinical and Laboratory Standards Institute (CLSI) 2020
guidelines. However, for susceptibility to Colistin, the agar dilution method was performed.
Results: In our study, fifty-five Carbapenem-resistant Pseudomonas aeruginosa were isolated. Out of these fifty-five isolates,
thirteen (23.6%) were sensitive to Tazocin, seven (12.7%) were sensitive to Ceftazidime, fourteen (25.4%) were sensitive to
Gentamicin, 7(12.7%) were sensitive to Cefepime, (12.7%) to Aztreonam, 10(18.2%) were sensitive to Amikacin, 7(12.7%) to
Ciprofloxacin, 6(11%) to Levofloxacin and forty-two (76.4%) were sensitive to Colistin.
Conclusion: The susceptibility of Carbapenem-resistant Pseudomonas aeruginosa to all the antibiotics tested in this study was
low. Isolates displayed the highest susceptibility to Colistin
