Restoration of Germinal Epithelium with Usage of Antioxidant Oils in Phenytoin Induced Testicular Toxicity: A Comparative Study in Rats

Abstract

Objective: To evaluate antioxidant effect of Virgin Coconut Oil (VCO) and Corn Oil (CO) on germinal thickness (GT) in rats induced with phenytoin toxicity. Study design and setting: An Experimental Longitudinal study was done at AL-Tibri Medical College and Hospital Isra University Karachi Campus. Methodology: 28 male albino rats were selected and were divided into 4 groups each consisting of 7 rats. Group A received normal saline solution only, Group B received 10mg/kg/body weight of Phenytoin, Group C received VCO+ Phenytoin, and Group D received CO+ Phenytoin. Animals were euthanized on the 4th, 5th, & 6th week and tissue sample was acollected for measuring GT using stage micrometer. Data was analyzed using SPSS 20.0. All values were expressed as mean ± S.D., groups compared using One way ANOVA followed by Post hoc Tukey’s test with p- value <0.05. Results: on 4th, 5th, & 6th week significant difference in germinal thickness was seen in Group A comparing it against the phenytoin group B (P-value: <0.001, <0.001, & 0.001). On 4th, 5th, & 6th week significant difference in GT was seen in Group C (VCO + Phenytoin) comparing it against the phenytoin group B (P-value: <0.001, <0.001, &< 0.001). No significant difference in germinal thickness was seen on 4th, 5th, & 6th week was seen when Group D (CO + Phenytoin) was compared to Group B (p-value: 0.551, 0.954, & 0.931). Conclusion: Virgin Coconut Oil can reduce the negative effect caused by phenytoin induced toxicity by preventing thinning of the germinal layer.