Estimation of Oxidant and Antioxidant Levels in Hemodialysis Treated end Stage Renal Disease Patients and Comparison with Normal Population

Abstract

Objectives: To estimate the plasma oxidant and antioxidant levels in hemodialysis treated end stage renal disease patients and to match them with normal population. Study Design and Setting: It was a comparative prospective study. This study was carried out at Jinnah Post Graduate Medical Center Karachi, from January 2018 till December 2018. Methodology: The hemodialysis patients (group B) were selected from the Dialysis Center of JPMC Karachi whereas the controls (group A) were recruited from normal healthy population. Consecutive sampling technique was used. The cases taken were receiving maintenance hemodialysis thrice a week. Antioxidant levels were determined by estimating plasma superoxide dismutase (SOD) while oxidant levels were estimated by detection of serum malondialdehyde (MDA) and compared with the levels of control group. Results: The mean superoxide dismutase level in control group was 108.53±19.44 while mean SOD levels in hemodialysis group was 46.20±19.18. . In control group mean MDA was 10.87±3.04, and in hemodialysis group mean MDA was 31.01±8.48.This results show the increased risk of oxidative stress resulting in complications in hemodialysis patients. Conclusion: Antioxidant levels are reduced in hemodialysis patients as compared to the normal population while the oxidant levels are much increased in hemodialysis group. This imbalance contributes to the oxidative stress related complications taking place in these patients. This study will help the nephrologist to elaborate the protective role of antioxidant administration in oxidative stress that can improve the cardiovascular mortality rate in hemodialysis treated end stage renal disease