DETERMINATION OF BACTERIAL DIVERSITY IN ANAEROBIC COMPLETE MIX BIOREACTOR FOR TREATMENT OF SYNTHETIC DOMESTIC WASTEWATER

Abstract

Bacteria play an important role in wastwater treatment as they decompose complex materials in simplest form.All life forms rely on bacterial processes for their survival. Bacterial diversity is greater than the diversity of any other organisms because bacteria play an important role in metabolic functions that affect soil, plant and human health. The objective of this study was to determine bacterial diversity in anaerobic complete mix bioreactor for treatment of synthetic wastewater. Sludge samples from I-9 sewage treatment plant was used as seed in the reactor.In the present study different bacterial species were identified from isolation and identification.In this study different techniques were used for the isolation and identification of bacteria. Those techniques were colony morphology, and biochemical test were performed such as Gram staining, catalase test, citrate test ,oxidase test .The result showed that different bacterial species were isolated with respect to the colony morphology most colonies were circular. paired, irregular, filamentous. For identification of species the isolated and purifying colonies were shipped to Microgen for characterization and amplification of 16sRNA gene sequencing and the dominants species are Acinetobacter, salmonella, Aeromonas spp, Citrobacter. Clostridium , Salmonella sp, Bacillus Clostridiumsp and uncultured bacteria. This study play an important role in identification and isolation of bacterial communities which are involved in treatment of domestic wastewater and the effect of mixing speed on microbial profiling and also phylogenetic analysis of microbes which are playing an important role in wastewater treatment anaerobically.