Abstract:
In the present study, bacterial strains were isolated from wastewater samples of metal
polluted environment. As a result, various 164 heterotrophic bacterial strains were isolated.
Out of them only Bacillus sp. Is studied the multiple metal tolerance profile and
bioaccumulation potentiality. We also analyze the metal contamination of selected area. The
average abundance order heavy metal content in water sample was Zn>Pb. Zinc
concentration was range from 39.832µg/L to 310.24 µg/L in water on the other hand lead
concentration was 8.09µg/L to 25.23µg/L in water. Metal resistance studies of the bacterial
isolates revealed that out of 164 isolates collected, bacillus sp. show tolerance to lead that
was more than 6000µg/ml. Zinc tolerance was range around 500µg/ml. Bioaccumulation
studies shows that with increase in time, biomass of selected strain also increase.
Correspondingly, with increase in biomass, the heavy metal bioaccumulation of that selected
bacterial strain also increase. In lead removal studies, around 10% of lead in experimental
flask was absorbed by Bacillus sp. I zinc removal studies, about 13% of zinc was reduced
in experimental flask. The obtained results showed that the selected Bacillus sp. is good
bioaccumulation strain for lead and zinc ions.
