COMPARATIVE STUDY OF TECOMELLA UNDULATA’S EFFECT AND N-ACETYLCYSTEINE ON PARACETAMOL INDUCED LIVER DAMAGE IN ALBINO RATS

Abstract

This study evaluated the hepatoprotective potential of Tecomella undulata bark extract on Paracetamol induced hepatotoxicity in rats in comparison with N-Acetylcysteine. This study was conducted at BMSI animal house. This was an experimental study with sample size (n=56) healthy Albino rats weighing 150-200gm. Experimental animals were divided into 4 groups with 14 animal in each group. Group A animals were controlled, Group B animals were induced Paracetamol intoxication at dose of 2 gm/kg body weight, orally as a single dose. Group C animals were intoxicated with Paracetamol and were treated with standard drug N-acetylcysteine at dose of 140mg/kg body weight, I/P for 6 days and Group D animals were treated with Tecomella plant extract at the dose of 200 mg/Kg bodyweight for 15 day, after Paracetamol intoxication. Animals were sacrificed at day 15 and blood samples were collected for Alkaline phosphatase, Alanine amniotransferase and Aspartate aminotransferase. Liver specimens were sent for histopathological observation for each group. Data was analyzed using SPSS version 23. ANOVA was applied. P-value <0.05 was considered significant. Serum liver enzyme levels of different groups were compared to see the hepatoprotective effect of Tecomella plant extract in Paracetamol intoxicated rat and rats treated with N-acetylcysteine. Histopathological hepatic cell changes were observed in all groups to check the hepatoprotective potential of Tecomella plant extract. Tecomella plant extract demonstrated its hepatoprotective effect by normalizing the liver enzyme levels and maintaining normal histoarchitecture of hepatocyte in Paracetamol induced liver damage in rats as compared to N-acetylcysteine. Hence, the plant extract would be an alternate antidote to N-acetylcysteine in Paracetamol induced hepatotoxicity in rats